As documented in 1566 during the pastoral visit of Saint Charles Borromeo, the Church of Saints Donato and Carpoforo has occupied this site since at least the 16th century. It later became a significant site of Marian devotion, known as the Sanctuary of the Blessed Virgin of the Rosary, housing an imposing late-16th-century wooden statue of the Madonna and Child, now positioned at the center of the apse between the two patron saints. The modest structure features a single nave and an apse with frescoed vaults; the panels, set within illusionistic architectural frames, depict the Mysteries of the Rosary (Joyful, Sorrowful, and Glorious). Behind the altar, light filters through symmetrical stained-glass windows depicting the Annunciation. The decorative scheme includes a fresco on the left column showing the Virgin and Child with an angel rescuing souls from Hell, while the right column portrays the preaching of Saint Anthony. Along the nave walls, large late-19th-century canvases represent the Apostles holding scrolls of the Creed. Outside, a square bell tower stands beside the church, which overlooks a raised churchyard. The cult of the Rosary, dating back to the 13th century and spread by the Dominicans, is solemnly celebrated at the Sanctuary of Trezzano Rosa every first Sunday of October.